Nyocha nyocha nke ihe nlele mmiri nwere ọtụtụ ngwa na sayensị ndụ na nlekota gburugburu ebe obibi.N'ime ọrụ a, anyị emepụtala fotometer kọmpat na nke dị ọnụ ala dabere na metal waveguide capillaries (MCCs) maka mkpebi siri ike nke absorption.Enwere ike ịbawanye ụzọ anya anya nke ukwuu, yana ogologo oge karịa ogologo anụ ahụ nke MWC, n'ihi na ọkụ gbasasịrị site na mgbidi metal dị larịị nwere ike ịdị n'ime capillary n'agbanyeghị akụkụ nke ihe kpatara ya.Enwere ike nweta nlebara anya dị ka 5.12 nM site na iji reagents chromogenic nkịtị n'ihi nkwalite ngwa anya ọhụụ na-abụghị nke linear yana mgbanwe nlele ngwa ngwa yana nchọpụta glucose.
A na-eji fotometry eme ihe maka nyocha nyocha nke ihe nlele mmiri n'ihi ụbara nke reagents chromogenic dị na ngwaọrụ optoelectronic semiconductor1,2,3,4,5.Tụnyere mkpebi nnabata nke dabere na cuvette, mmiri mmiri waveguide (LWC) capillaries na-egosipụta (TIR) site na idobe ìhè nyocha n'ime capillary1,2,3,4,5.Otú ọ dị, na-enweghị ọganihu ọzọ, ụzọ ngwa anya dị nso na ogologo anụ ahụ nke LWC3.6, na ịba ụba ogologo LWC gafere 1.0 m ga-ata ahụhụ site na attenuation ọkụ siri ike na nnukwu ihe ize ndụ nke egosipụta, wdg 3, 7. na cell multi-ntụgharị uche a tụrụ aro maka ndozi ụzọ anya, njedebe nchọpụta na-eme ka ọ dịkwuo mma site na 2.5-8.9.
E nwere ụdị isi abụọ nke LWC ugbu a, ya bụ Teflon AF capillaries (nwere akara ngosi nke naanị ~ 1.3, nke dị ala karịa nke mmiri) na silica capillaries kpuchie na Teflon AF ma ọ bụ ihe nkiri metal1,3,4.Iji nweta TIR na interface dị n'etiti ihe dielectric, ihe ndị nwere ihe ntinye aka dị ala na akụkụ ọkụ dị elu dị mkpa 3,6,10.N'ihe gbasara Teflon AF capillaries, Teflon AF na-eku ume n'ihi nhazi ya na-adịghị mma3,11 ma nwee ike ịmịnye obere ihe na mmiri.N'ihi na quartz capillaries mkpuchi n'èzí na Teflon AF ma ọ bụ metal, refractive index nke quartz (1.45) dị elu karịa ọtụtụ mmiri mmiri samples (eg 1.33 maka mmiri) 3,6,12,13.Maka capillaries nke ejiri ihe nkiri metal kpuchie n'ime, a na-amụ ihe ndị na-ebugharị 14,15,16,17,18, ma usoro mkpuchi ahụ dị mgbagwoju anya, elu nke ihe nkiri ígwè ahụ nwere usoro siri ike na nke porous4,19.
Na mgbakwunye, LWC ndị azụmahịa (AF Teflon Coated Capillaries na AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) nwere ụfọdụ ọghọm ndị ọzọ, dị ka: maka mmejọ..Nnukwu olu nwụrụ anwụ nke TIR3,10, (2) njikọ T (iji jikọọ capillaries, fibers, na inlet/outlet tubes) nwere ike ọnyà ikuku10.
N'otu oge ahụ, mkpebi siri ike nke ọkwa glucose dị oke mkpa maka nchọpụta ọrịa shuga, imeju imeju na ọrịa uche20.na ọtụtụ ụzọ nchọpụta dị ka photometry (gụnyere spectrophotometry 21, 22, 23, 24, 25 na colorimetry na akwụkwọ 26, 27, 28), galvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, anya polarimetry 3. elu plasmon resonance.37, Fabry-Perot oghere 38, electrochemistry 39 na capillary electrophoresis 40,41 na na.Agbanyeghị, ọtụtụ n'ime ụzọ ndị a chọrọ akụrụngwa dị oke ọnụ, yana ịchọpụta glucose n'ọtụtụ ọnụọgụ nanomolar ka bụ ihe ịma aka (dịka ọmụmaatụ, maka nha fotometric21, 22, 23, 24, 25, 26, 27, 28, ọkwa glucose kacha ala).mmachi ahụ bụ naanị 30 nM mgbe ejiri ihe nanoparticles blue Prussian mee ihe dị ka peroxidase mimics).A na-achọkarị nyocha glucose nke Nanomolar maka ọmụmụ ihe ọmụmụ cellular dị ka mgbochi nke prostate cancer prostate 42 na omume ndozi CO2 nke Prochlorococcus na oke osimiri.
Oge nzipu: Nov-26-2022